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Budownictwo i Inżynieria Środowiska

Budownictwo i Inżynieria Środowiska
15/135, DOI: 10.7862/rb.2015.135

NEW METHOD FOR EVALUATION OF THE MICROBIOLOGICAL QUALITY OF WATER

Justyna ZAMORSKA, Dorota PAPCIAK, Monika ZDEB

DOI: 10.7862/rb.2015.135

Abstract

Determination of the microbiological quality of water is one of the central tasks of environmental engineering. Reliability and accuracy of the results depends largely on the sampling system and the methodology carried signs. Typically, they are used traditional indirect methods, breeding: plate method or test tube fermentation. In the methods, seed stocks, it is assumed that each individual cell of bacteria transferred to a solid substrate colony grows. Thus, in fact, the result of the study determines the number of units capable of forming colonies, which is not equivalent to the amount of the living. Even larger errors are subject to the results of the analysis of test tube fermentation, which can only be the most probable number of bacteria in a given volume. It is therefore only estimating the number of microorganisms. Moreover, indirect methods are extremely time consuming (about 1 - 7 days of). Flow cytometry is an analytical technique that allows for the rapid measurement of scattered light and fluorescence signals emitted by cells exposed respectively. Allows for a qualitative and quantitative assessment of the physical and biological properties of the cells in a short time. The article presents the results of research on the amount of bacterial cells in different water: surface, groundwater, rainwater and tap water using flow cytometry and compare this results obtained with traditional methods of reference. The microbial results in all the tested waters by flow cytometry are much higher than the amount of the reported conventional methods.

Full text (pdf)

References

[1]    Chełmicki W., Water - resources, degradation, conservation, PWN, Warszawa 2012 (in polish).

[2]    Dojlido R., Ecology and Environment, PWN , 1997 (in polish).

[3]    Egli T., Eawag News 2008, Nr 65e, New methods for assessing the safety of drinking water, s. 20-22.

[4]    Hammes F, Berney M, Wang Y, Vital M, Köster O, Egli T.,Flow-cytometric total bacterial cell counts as a descriptive microbiological parameter for drinking water treatment processes. Water Res., 2008, 42, s. 269-277.

[5]    Hammes F., Egli T., Cytometric methods for measuring bacteria in water: advanteges pitfalls and application, Anal.Bioanal.Chem., 2010, 397, s. 1083-1095.

[6]    Hyka P., Lickova S., Pribyl P., Melzoch K., Kovar K., Biotechnology Advances, JBA – 06582, Flow cytometry for the development of biotechnological processes with microalgae, 2012, s. 3-8.

[7]    Pawlaczyk-Szpilowa M., Exercise of water and wastewater microbiology, PWN, Warszawa 1980 (in polish).

[8]    Prest E. I., Hammes F., Kotzsch S., M.C.M. van Loosdrecht, Vrouwenvelder J. S., Water Research, 47, Monitoring microbiological changes in drinking water systems using a fast and reproducible flow cytometric method, 2013, s. 7131-7134.

[9]    Sędek Ł., Sonsala A., Szczepański T., Mazur B., Technical aspects of flow cytometry, Journal of Laboratory Diagnostic 2011, s. 415-417.

[10] Skotny A., Pucińska J., Modern flow cytometry, Biomedical engineering, 2013, s. 4 – 8.

[11] Siebel E., Wang Y., Egli T., Hammes F., Correlation between total cell concentration, total adenosine tri-phosphate concentration and heterotrophic plate counts during microbial of drinking water, Drinking Water Engineering and Science, 2008, 1, s. 1-6.

[12] Walczak M, i inni, Fundamentals of microbiology in theory and practice, Scientific Publishing Nicolaus Copernicus University, Toruń 2013 (in polish).

About this Article

TITLE:
NEW METHOD FOR EVALUATION OF THE MICROBIOLOGICAL QUALITY OF WATER

AUTHORS:
Justyna ZAMORSKA (1)
Dorota PAPCIAK (2)
Monika ZDEB (3)

AUTHORS AFFILIATIONS:
(1) Rzeszów University of Technology
(2) Rzeszów University of Technology
(3) Rzeszów University of Technology

JOURNAL:
Budownictwo i Inżynieria Środowiska
15/135

KEY WORDS AND PHRASES:
water bacteriology, bacterial plate count, drinking water, flow cytometry

FULL TEXT:
http://doi.prz.edu.pl/pl/pdf/biis/306

DOI:
10.7862/rb.2015.135

URL:
http://dx.doi.org/10.7862/rb.2015.135

COPYRIGHT:
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